Tired of her rural home, recent college graduate Yoshino Koharu is desperate to lead a more exciting life in Tokyo. After a fruitless job hunt, she finally receives a part-time offer as queen of the bizarre Kingdom of Chupakabura, a rundown mini-attraction in the small agricultural town of Manoyama. However, Yoshino discovers upon her arrival in Manoyama that she was mistaken for a celebrity and the job offer was a mistake. Left with no other options, Yoshino reluctantly agrees to take on the role and aid the Board of Tourism in their efforts to revitalize Manoyama. Determined to bring excitement to the dying town with the help of local residents, the queen enacts a series of projects to highlight the beauty and charm of Manoyama's culture. Sakura Quest delves into the story of a tight-knit community that is struggling to balance change while also maintaining the rich traditions and bonds which define their identity.

Otokojuku, a private school for juvenile delinquents that were previously expelled from normal schools. At this school, Japanese chivalry is taught through the feudal and military fundamentals. Similar to an action film, the classes are overwhelmed by violence. Only those who survive it become true men. (Source: Animenfo)

The Achiga Girls' Academy in Nara once defeated regional mahjong powerhouse Bansei High School. It advanced into the national team semifinals but lost to the eventual champion, and the mahjong club was later disbanded. Six years later, elementary school student Shizuno Takakamo befriends transfer student Nodoka Haramura. The two eventually enter Achiga Girls', but Nodoka transfers out of the school in the second year. When Shizuno sees Nodoka on television the following year as the national middle school individual mahjong champion, she decides to revive Achiga's mahjong club.

Despite leading a peaceful life as the princess of a subterraneous colony, teenage Patema longs to escape her cramped industrial confines and experience someplace different. While satiating her curiosity by exploring a region known as the danger zone, she is startled by a cloaked figure and falls into the darkness of an abyss. Awakening with the sky below her feet and the ground hanging above, Patema finds herself clinging to a fence at the mercy of a seemingly reversed gravity. Although a nearby student named Age is able to rescue her, they are left wondering which of the two is actually upside down.