The movie begins with Fuyuki Hinata and the Keroro Platoon exploring an abandoned temple in the mountaintop city of Machu Picchu in Peru. While on the exploration, Keroro accidentally sets off a trap, and by chance they find a hidden chamber with a large blue crystal in the center, on a stand that has a key in it. Keroro leans on the key, causing the room to activate a mysterious machine, this caused the entire temple to shake. As soon as the group realized what was happening, they run out of the room. While running, Keroro accidentally loses his Kero Ball while Fuyuki catches a fleeting glimpse of a glowing young woman as he is running out, and tells Keroro that there was a girl in the room, but he is forced to leave the room before he can say anything else. The group successfully escapes, while the girl (now sprouting a pair of glowing wings) watches them leave - while back in the chamber, something is growing within the crystal... (Source: AniDB)

One day, a stone carving bearing an uncanny likeness to Keroro arrives at Fuyumi's place. To solve this mystery, the Keroro Squad travels to the isolated and mysterious Easter Island. There, they meet harmless spirits known as Mana, and the odd twins Io and Rana. Lastly, there is Akuaku, a legendary evil spirit recently awoken from its long sleep. (Source: AniDB)

Telegraph Pole Society, a mysterious group, is planning to conquer the world. The principal of the local high school forms a Defense Force by recruiting students from school: Shogi Morita, Kuho Tasuke, and Akiko Ifukube. Meanwhile the high school crazy scientist, Dr. Inogami, has turned a local boy, Kami Sanchin, into a cyborg. They might just defeat the Telegraph Pole Society with the help of the cyborg, or maybe not... (Source: AnimeNfo)

Battles continue between Penguin Empire, the mysterious life form which arrived on earth all of a sudden, and Beauty Salon Prince, the secret anti-Penguin organization. In order to fight Penguin Empire's gigantic Penguin Robot, Beauty Salon Prince has developed their ultimate weapon, Daimidaler, and is now looking for the pilot. They choose Koichi Madanbashi, a hot-blooded high school boy who is mad about big boobs, because he is the factor of Hi-ERo particles, the source of unknown energy. (Source: Showgate)