In the light of day and in the dead of night, mysterious horrors await in the darkest shadows of every corner. They are unexplainable, inescapable, and undefeatable. Be prepared, or you may become their next victim. Sit back in terror as traumatizing tales of unparalleled terror unfold. Tales, such as that of a cursed jade carving that opens holes all over its victims' bodies; deep nightmares that span decades; an attractive spirit at a misty crossroad that grants cursed advice; and a slug that grows inside a girl's mouth. Tread carefully, for the horrifying supernatural tales of the Itou Junji: Collection are not for the faint of heart.

Mai, a Santa in training, appears in front of an unlucky boy named Santa on Christmas Eve, promising him that she will make him happy for one night.

At Okagi Castle, Yuuki informs his childhood friend An that his family is moving away. Vowing to return when he is older, Yuuki confesses that he would like them to go out when that happens. Unsure if their feelings for each other will remain the same when they next meet, Ann runs off in a panic, only to encounter a portal at the entryway of the castle. Stepping through it, she finds herself 50 years in the future. In her confusion, An is approached by a boy named Kibou and his friendly robot, Butler-kun. As luck would have it, Kibou's grandmother instructed him to lend a hand to any troubled girls he encounters at the park, so he offers to be her chaperone. As the three explore the city, An notices that despite how much Okagi has changed, the old charm never evaporated from the nostalgic townscape.

Every man wants to see panties, but unfortunately, that happens to be difficult to achieve. They are hidden beneath layers of clothing and seldom shown, other than to the trustworthy. They are considered the final frontier and the last barrier between a woman's bare skin and the wider world. The moment a girl shows her panties is when she is the most vulnerable. It would be absurd to simply ask to see them—or would it? If a variety of girls were brazenly asked to show their panties, they would naturally be filled with contempt. But if one were to beg hard enough, they just might be lucky enough to get a peek.