The world is dying, no longer able to support life. The Ainsworth family can use the Holy Grail to save humanity, but the price of salvation is sacrifice of the magical girl Miyu — but Miyu’s brother, Shirou, won’t let them have her without a fight. Now it’s up to Illya to step in and find a solution that will save not only Miyu, but the entire world from impending doom. But when the price for the world's salvation is the life of a single, innocent girl, what will Illya choose? (Source: Sentai Filmworks)

Coming from a renowned magus family, Ayaka Sajou is much expected to partake in the Holy Grail War. But ever since her father and sister's demise in the previous battle, she has grown gloomy and unwilling to involve herself in the matters that killed her loved ones. Soon, she finds herself in grave danger at the hands of a knight and ends up summoning her own Servant named Saber. With him by her side, Ayaka ultimately becomes an official participant in the war she initially wanted to avoid so much.

The Holy Grail War: a violent battle between mages in which seven masters and their summoned servants fight for the Holy Grail, a magical artifact that can grant the victor any wish. Nearly 10 years ago, the final battle of the Fourth Holy Grail War wreaked havoc on Fuyuki City and took over 500 lives, leaving the city devastated. Shirou Emiya, a survivor of this tragedy, aspires to become a hero of justice like his rescuer and adoptive father, Kiritsugu Emiya. Despite only being a student, Shirou is thrown into the Fifth Holy Grail War when he accidentally sees a battle between servants at school and summons his own servant, Saber. When a mysterious shadow begins a murderous spree in Fuyuki City, Shirou aligns himself with Rin Toosaka, a fellow participant in the Holy Grail War, in order to stop the deaths of countless people. However, Shirou's feelings for his close friend Sakura Matou lead him deeper into the dark secrets surrounding the war and the feuding families involved.

In Fuyuki City, the Fifth Holy Grail War is about to commence a lengthy battle of blood, death, and misery. High school student Rin Toosaka has trained her entire life for this moment—to become a magus capable of being a Master in the war. Summoning her Servant known as Archer, Rin finally sets foot into the battle. Discovering that one of her acquaintances, Shirou Emiya, is drawn into the war as well, Rin offers to form an alliance for the time being. And as the two of them grow closer, Rin begins to learn more about Shirou's fate and ideals. Nevertheless, her goal remains the same: to win the all-powerful relic that can fulfill the wishes of those who are victorious after the war—the Holy Grail.