Second Lieutenant Kei Agemaki, the son of a famous general, has hidden his extreme fear of paranormal beings all his life. However, when he and two others are reassigned to live and work with youkai in the Ministry of Spirit Affairs, he is brought face-to-face with his worst nightmare. Now with the help of the fox spirit Kushimatsu, he and his fellow officers must learn to work alongside youkai maidens—Zakuro, Susukihotaru, Hoozuki, and Bonbori—to solve paranormal cases. Set in the midst of an alternate version of Japanese Westernization, Otome Youkai Zakuro explores the clashes and unions that can occur when east meets west, local meets foreign, and women meet men. The unusual alliance of the youkai maidens and human officers must learn to work together in a world that is changing around them.

The principal character is 15-year-old Isabelle Laustin, other characters being her parents Leon and Marie, Isabelle's friends Jean and his sister Marie, and Isabelle's suitor, Captain Victor. When Paris is besieged and Napoleon III's army is defeated, Isabelle escapes to London, disguised as a boy, and engages in secret agent stuff. (Source: AniDB)

The anime will feature an original story from the game on which it is based.

Although traumatized by witnessing the murder of his parents by one of the Choushuu, Ichimura Tetsunosuke's thirst for revenge leads him to desire strength. At the age of 15, Tetsunosuke approached the Shinsengumi, wanting to become one of its members. However, Tetsunosuke lacked the skill, mind, and will to emotionlessly cut down whoever threatened peace and the Shinsengumi. Even with the support of his brother Tatsunosuke and his newfound friends of the Shinsengumi, little did Tetsunosuke know the blood and pain he would have to face being part of this historical group.