Six Gravity and Procellarum, Tsukino Talent Productions' best male idol groups, are preparing for this year's edition of the Full Moon Festival. The show's theme is a new self, which seems to excite all the members. Some of the members from both units struggle to find inspiration for the theme, having matured from their last festival appearance. As they continue their daily lives while juggling their show business responsibilities, each of the Six Gravity and Procellarum members discover the meaning of a new self.

For the members of SolidS, Growth, SOARA, and QUELL, the Tsukino Talent Productions' Live 2021 Summer Carnival is coming soon. The two-day event will culminate with a joint live performance featuring all four units. Due to the size and setup of the stage, each unit will need to create new songs and choreographies to accompany them in the riveting second season of Tsukipro The Animation.

At the Budokan, an annual event known as the Tsukino Concert puts Tsukino Entertainment Production's best and brightest idol units on the stage. The leaders of idol groups SolidS, SOARA, QUELL, and Growth must compose four unique and distinctive songs for their respective units, which they will perform on the second day of the festival. Composing four new songs proves to be a difficult task for SolidS's leader, Shiki Takamura, as he struggles to find inspiration. Sora Oohara must learn to overcome past mistakes while propelling his unit, SOARA, to the top. Wanting to escape his past stardom, Shuu Izumi is determined to push his unit, QUELL, up to the level of popularity that his previous one had. Growth finds themselves working together with a rival group, ZIX, prior to their debut—something that leader Kouki Etou is reluctant to do. Tsukipro The Animation tells the stories of these four units simultaneously as they go through their daily lives while preparing for the Tsukino Concert.