After transferring to Ishinomori Middle School, Tetsuko Alice Arisugawa overhears her classmates discussing a strange rumor. The previous occupant of her seat, a nondescript Judas, is missing and presumed murdered—poisoned by one of his four wives. Amidst dealing with her mother's undesired meddling in her affairs and warding off her classmates' bullying, the troubled Alice starts to investigate the mystery surrounding Judas' disappearance. The trail of clues leads her to the house of her next-door neighbor, the quirky and reclusive Hana Arai. After an awkward introduction, Hana agrees to help Alice and formulates a plan that may lead them to the answers they seek. The two girls embark on a journey to uncover what happened to Judas, and perhaps a friendship may start to blossom between the two along the way.

After contracting hepatitis A, Ezaki Yuuichi has been confined to a hospital, away from his friends and family, much to his displeasure. To relieve his boredom, he has taken to sneaking out of the hospital, usually putting himself on the receiving end of a beating from his nurse. Upon meeting a girl his age also staying in the hospital, he is immediately captivated by her beauty. Akiba Rika's personality is not quite as captivating as her beauty however. In fact, she is rather selfish, moody, and bossy. But as the two spend more time with each other, they become closer, sharing the ordinary joys and trials of a budding teenage romance, even when darkened with impending tragedy—for Rika's condition does not leave her much longer to live.

Ohana Matsumae is an energetic and wild teenager residing in Tokyo with her carefree single mother. Abruptly, her mother decides to run away with her new boyfriend from debt collectors, forcing the young girl to fend for herself—as per her mother's rely only on yourself philosophy—in rural Japan, where her cold grandmother runs a small inn. Driven to adapt to the tranquil lifestyle of the countryside, Ohana experiences and deals with the challenges of working as a maid, as well as meeting and making friends with enthralling people at her new school and the inn.

Striking fear into the hearts of mortals, the Demon Lord Satan begins to conquer the land of Ente Isla with his vast demon armies. However, while embarking on this brutal quest to take over the continent, his efforts are foiled by the hero Emilia, forcing Satan to make his swift retreat through a dimensional portal only to land in the human world. Along with his loyal general Alsiel, the demon finds himself stranded in modern-day Tokyo and vows to return and complete his subjugation of Ente Isla—that is, if they can find a way back! Powerless in a world without magic, Satan assumes the guise of a human named Sadao Maou and begins working at MgRonald's—a local fast-food restaurant—to make ends meet. He soon realizes that his goal of conquering Ente Isla is just not enough as he grows determined to climb the corporate ladder and become the ruler of Earth, one satisfied customer at a time! Whether it's part-time work, household chores, or simply trying to pay the rent on time, Hataraku Maou-sama! presents a hilarious view of the most mundane aspects of everyday life, all through the eyes of a hapless demon lord.