Six Gravity and Procellarum, Tsukino Talent Productions' best male idol groups, are preparing for this year's edition of the Full Moon Festival. The show's theme is a new self, which seems to excite all the members. Some of the members from both units struggle to find inspiration for the theme, having matured from their last festival appearance. As they continue their daily lives while juggling their show business responsibilities, each of the Six Gravity and Procellarum members discover the meaning of a new self.

At the Budokan, an annual event known as the Tsukino Concert puts Tsukino Entertainment Production's best and brightest idol units on the stage. The leaders of idol groups SolidS, SOARA, QUELL, and Growth must compose four unique and distinctive songs for their respective units, which they will perform on the second day of the festival. Composing four new songs proves to be a difficult task for SolidS's leader, Shiki Takamura, as he struggles to find inspiration. Sora Oohara must learn to overcome past mistakes while propelling his unit, SOARA, to the top. Wanting to escape his past stardom, Shuu Izumi is determined to push his unit, QUELL, up to the level of popularity that his previous one had. Growth finds themselves working together with a rival group, ZIX, prior to their debut—something that leader Kouki Etou is reluctant to do. Tsukipro The Animation tells the stories of these four units simultaneously as they go through their daily lives while preparing for the Tsukino Concert.

On November 23, 1957, the whole world witnessed the Federal Republic of Zirnitra's monumental achievement of sending the first live animal—a dog—to outer space. Since then, the space race between the confederacy and its competitor, the United Kingdom of Arnack, has intensified; the two countries hope to one day send humans to the cosmos above. As a dog's biology is inherently different from a human's anatomy, there is no way to perfectly identify the risks involving space travel and its effects on an individual's body without actually sending someone for observation. However, Zirnitra's government has a potential solution: to experiment on vampires, whose biological similarity to humans is too significant to ignore. Despite being forcibly taken from her home in the mountains, vampire Irina Luminesk shows no resistance and is even willing to train as a test subject. Lev Leps, a former top candidate to become the first human cosmonaut, is designated to accompany Irina and act as her guide. Through their time together, Irina and Lev begin to develop a mutual love for outer space, bringing them closer together.

Tsuyoshi Ikawa lives at home with his popular and beautiful sisters. Despite their qualities, his sisters and his mother are horrible at household chores, including cooking, so all these duties are taken over by Tsuyoshi while his father is away due to being temporarily transferred to another city for work.