The long-awaited finals of the Spring Tournament have arrived for Karasuno High School's volleyball team. The winner of the match will go on to represent all of Miyagi Prefecture at the National Tournament. However, the last challenge Karasuno must overcome is to win against Shiratorizawa Academy, the school that houses Wakatoshi Ushijima, one of Japan's top three aces. Shiratorizawa is the reigning Miyagi champion for the past three years, and Karasuno seems to have little chance at dethroning them. When the other school's captain Ushijima insults Karasuno to their faces, freshmen duo Shouyou Hinata and Tobio Kageyama swear to defeat them and steal their spot in the nationals. Against a roster full of nationally-ranked players, the former flightless crows will have to use everything in their arsenal if they want to represent Miyagi.

After watching a volleyball match on TV where the Little Giant of Karasuno High School helps his team secure a hard-fought victory, young and impressionable Shouyou Hinata begins to play the sport in hopes that he too can soar high one day. Now enrolled at the same school as his idol, he attempts to join the volleyball team despite his short stature. Unfortunately, upon his arrival, Hinata locks eyes with King of the Court Tobio Kageyama—a genius setter whom he had once suffered a bitter defeat to in middle school. Their unfriendly introduction and subsequent rivalry forces the rest of the Karasuno team to bar both of them from joining the club until they can learn to work together as teammates. Though reluctant to cooperate, their desire to play volleyball causes them to return with a proposal that will show the other members that they are capable of teamwork. As the two freshmen form an odd setter-and-ace relationship, an old rival school invites Karasuno for practice matches, reigniting competition between the two schools once more.

The Inter-High has finally arrived for Karasuno High School's volleyball team. Their opponent at the tournament is Date Tech High School—the team that crushed Karasuno the year prior. The loss brings back anxiety and fear for the team's second- and third-years as they were unable to overcome Date's read block. With the dynamic freshmen duo Shouyou Hinata and Tobio Kageyama on their side, will Karasuno be able to overcome their past and break through the iron wall? Awaiting Karasuno in the next round is the elite Aoba Jousai High School, led by the cunning captain Tooru Oikawa. Coming from the same middle school as Kageyama, Oikawa is a talented and unpredictable setter with a grudge against his former teammate. It is a battle between genius setters to decide who will continue on in the Inter-High.

An intense battle rages on at the Tokyo Qualifiers for the three remaining spots in the national volleyball competition. Nekoma High School, Fukurodani High School, Nohebi Academy, and Itachiyama Academy all passionately strive to participate in the tournament. Despite various issues on the court, Nekoma especially wishes to prove they are worthy of moving on to the national level. As the teams aim to secure their place by overcoming both their opponents and their own weaknesses, the Tokyo Qualifiers determine which teams will reign victorious and join the national competition.