While taking a ferry to Fuuka Academy, new students Mai Tokiha and her sickly younger brother, Takumi, spot a girl adrift on the water. Shortly after bringing her aboard, the ferry comes under attack from an assailant who summons a mechanical monster. Despite still recovering from her injuries, Mikoto Minagi fends off the monster using a supernatural sword. Mai soon discovers that Mikoto is a fellow Fuuka Academy student with abilities called HiME (Highly-advanced Materializing Equipment). Female students with HiME are responsible for defending the academy and the local city from the monstrous Orphans. After several incidents at the school involving battles against these mysterious creatures, Mai learns that she is a HiME herself and is capable of summoning a Child, a mechanical creature capable of great utility and firepower. Now burdened with new responsibilities, Mai joins 12 other HiME as they safeguard the school from a threat they know little about all while protecting the ones closest to them.

Extra shorts that come after each episode. This entry also includes the Mai Hime vs. Mai Otome - Moe? The Greatest Battle! episode.

It's just another normal day at a maid café until one of the customers turn out to be a zombie! Disrupting business and infecting some of the maids, everything suddenly descends into chaos as people are attacked. As one of the more experienced maids comes to deal with the unruly customer and newly-made zombie maids, perhaps there's a bright side to this unbelievable turn of events after all?

Machiko Mai is the newest teacher at Asama Elementary; she's young, beautiful, and has the perfect proportions—traits that attract the attention of not only her students, but the rest of the perverted faculty as well. Whether it's luring her in and spraying up her skirt with a hose or leaps and bounds to grab her breasts, there's nothing that Machiko's first year students won't try to do. Regardless of her embarrassing plight, Machiko is always a good sport and tries her best to help out her students—at any cost to her dignity!