The competitive shinobi world has proven to be challenging for Rock Lee, who lacks the typically required abilities. To compensate for this handicap, the young ninja tenaciously endures severe training to hone his hand-to-hand combat skills. Whenever necessary, he also resorts to creative fighting techniques. With the constant support of his teammates Neji Hyuuga and Tenten, Lee readily embraces daring adventures as opportunities to advance on his path toward excellence.

Anzu Hoshino needs only three things in her life: video games, chocolate, and her beloved cat. Unlike other high school girls, Anzu has no time for or interest in romance. But as she begins playing a poorly programmed 3D otome game, a bizarre flying wizard named Riri emerges from the screen and calls Anzu subject one, the first person who will experience a dating game harem storyline in real life. Despite Anzu's fiery protests, Riri confiscates her favorite things to force her to focus on love. Riri orchestrates a series of unlucky incidents and romantic cliches that lead her to meet Tsukasa Kazuki, one of the most attractive boys in her school. Still enraged, Anzu is adamant about resisting Tsukasa's charm. As all the ridiculous fabricated scenarios help Anzu warm up to Tsukasa's pleasant nature, Riri throws other stereotypical pretty boys her way—and avoiding romance quickly becomes almost impossible.

It has been one year since Tsukune Aono enrolled at Youkai Academy, and since then his life has taken an interesting turn. In addition to being the only human at a school for monsters, he has attracted a bevy of beautiful women who want him all for themselves: the sexy succubus Kurumu Kurono; Yukari Sendou, a witch; stalker and yuki-onna Mizore Shirayuki; and Moka Akashiya, a kind vampire who, when her rosary is removed, reveals a darker personality. But soon, trouble visits Youkai Academy in the form of Moka's younger sister, Kokoa Shuzen. She is furious that her true older sister is sealed within Moka's rosary, and vows to bring out Moka's darker self. However, Kokoa's thoughtless actions may affect more than just her sister...

Youkai Academy is a seemingly normal boarding school, except that its pupils are monsters learning to coexist with humans. All students attend in human form and take normal academic subjects, such as literature, gym, foreign language, and mathematics. However, there is one golden rule at Youkai Academy—all humans found on school grounds are to be executed immediately! Tsukune Aono is an average teenager who is unable to get into any high school because of his bad grades. His parents inadvertently enroll him into Youkai Academy as a last-ditch effort to secure his education. As Tsukune unknowingly enters this new world, he has a run-in with the most attractive girl on campus, Moka Akashiya. Deciding to stay in the perilous realm in order to further his relationship with Moka, he does not realize that beneath her beauty lies a menacing monster—a vampire. Rosario to Vampire is a supernatural school comedy that explores Tsukune's romantic exploits, experiences, and misadventures with a bevy of beautiful but dangerous creatures.